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Detection of a single copy gene on a mitotic metaphase chromosome by fluorescence in situ hybridization (FISH) in the sawfly, Athalia rosae (Hymenoptera)
Author(s) -
Matsumoto Kouhei,
Yamamoto Daisuke S.,
Sumitani Megumi,
Lee Jae Min,
Hatakeyama Masatsugu,
Oishi Kugao
Publication year - 2002
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.10005
Subject(s) - sawfly , biology , hymenoptera , fluorescence in situ hybridization , metaphase , chromosome , fish <actinopterygii> , in situ hybridization , gene , zoology , genetics , gene expression , fishery
Mitotic metaphase chromosomes of Athalia rosae (Hymenoptera) haploid males were subjected to fluorescence in situ hybridization (FISH) analysis using an rDNA probe and two vitellogenin (Vg) cDNA probes (one representing the 5′ half and the other the 3′ half of the gene, each about 3 kb long, and together covering the entire coding region). The rDNA probe produced signals in four chromosomes, all in pericentromeric regions (haploid chromosome number = 8), and the Vg probes, either the combined probes or the 3′ region alone, produced a twin signal in the middle of a chromosome arm of a single chromosome. Arch. Insect Biochem. Physiol. 49:34–40, 2002. © 2002 Wiley‐Liss, Inc.