Open AccessComparative 35 S‐sulfate and 3 H‐proline metabolism within the interdental septal bone and adjacent periodontal ligament
Author(s) -
Johnson Roger B.
Publication year - 2006
Publication title -
the anatomical record part a: discoveries in molecular, cellular, and evolutionary biology
Language(s) - English
Resource type - Journals
eISSN - 1552-4892
pISSN - 1552-4884
DOI - 10.1002/ar.a.20343
Subject(s) - periodontal fiber , dental alveolus , interdental consonant , periodontium , bone remodeling , chemistry , anatomy , alveolar process , medicine , dentistry , endocrinology
Tooth movements require rapid remodeling of the periodontal ligament (PDL) and adjacent alveolar bone. Our objective was to compare the regional metabolism of sulfated‐glycosaminoglycans (sGAG) within the PDL and adjacent alveolar bone and compare it to the metabolism of collagenous proteins using radioautographic techniques. Rats were injected with either 3 H‐proline or 35 S‐sulfate and maxillae were removed at 1, 6, and 12 hr 1–7 days later. Silver grains were counted over the PDL and adjacent alveolar bone and the incorporation and removal rates for each radioisotope were determined. In general, net collagenous protein incorporation and removal were greatest within the distal and net sGAG incorporation and removal were greatest within the mesial compartments of the periodontium. The rate of removal of 3 H‐proline was significantly greater within the distal alveolar bone surface than the adjacent PDL at all levels ( P < 0.001). In contrast, the rate of removal of 35 S‐sulfate was significantly greater in the PDL than within the adjacent mesial surface of the interdental septum at all levels ( P < 0.001). The mesial surfaces of the interdental septum had a slower rate of removal of both isotopes than distal surfaces at all levels ( P < 0.001). Our data suggest significant regional differences in the metabolism of 35 S‐sulfate and 3 H‐proline within the PDL and alveolar bone, which likely result from the characteristics of the forces produced by the adjacent teeth and may be a factor in the remodeling of the alveolar wall coincident to tooth movement. Anat Rec Part A, 288A:817–826, 2006. © 2006 Wiley‐Liss, Inc.