Open AccessAT 4 receptor binding in the developing rabbit
Author(s) -
Burns Gilbert A.,
AnnMarie Thiessen,
Jodie Hanesworth,
Harding Joseph W.
Publication year - 2004
Publication title -
the anatomical record part a: discoveries in molecular, cellular, and evolutionary biology
Language(s) - English
Resource type - Journals
eISSN - 1552-4892
pISSN - 1552-4884
DOI - 10.1002/ar.a.20070
Subject(s) - fetus , receptor , rabbit (cipher) , sinus (botany) , binding site , chemistry , biology , anatomy , medicine , endocrinology , biophysics , biochemistry , pregnancy , zoology , genus , genetics , statistics , mathematics
The binding of the AT 4 ‐specific analog, divalinal‐AngIV (Dival), was studied in rabbit fetuses of various gestational ages. Saturation isotherm and competition data from selected tissues indicate that fetal Dival binding sites are saturable and specific for AT 4 ligands. Autoradiographs revealed that binding was present in all the specimens examined. The peripheral nerves, kidneys, and heart were particularly heavily labeled. Labeling of some tissues, such as forming bones, was not constant as gestational age increased. Other tissues, including multilocular fat, sinus hairs, and enamel organs of nascent teeth, exhibited substantial binding as these tissues developed. © 2004 Wiley‐Liss, Inc.