Open AccessExpression of cVg1 mRNA during chicken embryonic development
Author(s) -
Somi Semir,
Houweling Arjan C.,
Buffing Anita A.M.,
Moorman Antoon F.M.,
Van Den Hoff Maurice J.B.
Publication year - 2003
Publication title -
the anatomical record part a: discoveries in molecular, cellular, and evolutionary biology
Language(s) - English
Resource type - Journals
eISSN - 1552-4892
pISSN - 1552-4884
DOI - 10.1002/ar.a.10070
Subject(s) - biology , neural tube , messenger rna , heart development , notochord , primitive streak , embryogenesis , otic vesicle , mesoderm , embryo , paraxial mesoderm , retina , in situ hybridization , optic vesicle , anatomy , microbiology and biotechnology , gastrulation , embryonic stem cell , eye development , gene , genetics , neuroscience , phenotype
Using degenerated PCR‐primers to identify known and novel BMPs that are expressed in the developing chicken heart, we identified not only BMP2, ‐4, and ‐7 mRNA, but also the TGFβ superfamily member cVg1. The expression pattern of cVg1 mRNA was determined during chicken development from HH4 to HH44. In early developmental stages, cVg1 mRNA is expressed in the primitive streak, paraxial mesoderm, developing somites, and developing neural tube. Subsequently, cVg1 mRNA is expressed in the developing central and peripheral nervous system, retina, auditory vesicle, notochord, lung alveoli, and olfactory mucosa. In the heart, cVg1 is initially expressed through the linear heart tube, but becomes restricted to the forming chamber myocardium, in an expression domain similar to that of atrial natriuretic factor (ANF) mRNA. Anat Rec Part A 273A:603–608, 2003. © 2003 Wiley‐Liss, Inc.