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An in situ hybridization study of the Syndecan family in the developing condylar cartilage of fetal mouse mandible
Author(s) -
Fujikawa Kaoru,
Shibata Shunichi,
Nakamura Masanori
Publication year - 2021
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.24483
Subject(s) - syndecan 1 , cartilage , condyle , perichondrium , in situ hybridization , biology , anatomy , chondrogenesis , mesenchymal stem cell , mandible (arthropod mouthpart) , microbiology and biotechnology , cell , messenger rna , biochemistry , gene , botany , genus
Abstract Mandibular condylar cartilage is a representative secondary cartilage, differing from primary cartilage in various ways. Syndecan is a cell‐surface heparan sulfate proteoglycan and speculated to be involved in chondrogenesis and osteogenesis. This study aimed to investigate the expression patterns of the syndecan family in the developing mouse mandibular condylar cartilage. At embryonic day (E)13.0 and E14.0, syndecan‐1 and ‐2 mRNAs were expressed in the mesenchymal cell condensation of the condylar anlage. When condylar cartilage was formed at E15.0, syndecan‐1 mRNA was expressed in the embryonic zone, wherein the mesenchymal cell condensation is located. Syndecan‐2 mRNA was mainly expressed in the perichondrium. At E16.0, syndecan‐1 was expressed from fibrous to flattened cell zones and syndecans‐2 was expressed in the lower hypertrophic cell zone. Syndecan‐3 mRNA was expressed in the condylar anlage at E13.0 and E13.5 but was not expressed in the condylar cartilage at E15.0. It was later expressed in the lower hypertrophic cell zone at E16.0. Syndecan‐4 mRNA was expressed in the condylar anlage at E14.0 and the condylar cartilage at E15.0 and E16.0. These findings indicated that syndecans‐1 and ‐2 could be involved in the formation from mesenchymal cell condensation to condylar cartilage. The different expression patterns of the syndecan family in the condylar and limb bud cartilage suggest the functional heterogeneity of chondrocytes in the primary and secondary cartilage.