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Expression of Collagen Types I, II, IX, and X in the Mineralizing Turkey Gastrocnemius Tendon
Author(s) -
Taylor Reginald W.,
Mitchell Georgia K.,
Andrade Jon Lee,
Svoboda Kathy K.
Publication year - 2020
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.24091
Subject(s) - endochondral ossification , fibrocartilage , tendon , intramembranous ossification , chondrogenesis , extracellular matrix , chemistry , anatomy , type i collagen , cartilage , type ii collagen , matrix (chemical analysis) , mineralization (soil science) , pathology , biology , articular cartilage , medicine , osteoarthritis , biochemistry , alternative medicine , organic chemistry , chromatography , nitrogen
The turkey gastrocnemius tendon mineralizes by intramembranous ossification with a transient chondrogenic phase. The mineralizing zone has hypertrophic chondrocytes similar to endochondral bone formation. These similarities prompted the evaluation of this tendon for the presence of type X collagen in the mineralizing zone. Tendons were removed, radiographed, decalcified, and embedded for frozen sections. Seral sections were H&E stained and immunostained individually with antibodies specific collagens (types I, II, IX, and X). Type I collagen was distributed widely throughout the mineralized tendon extracellular matrix. Types II and IX collagen were at the mineralized/non‐mineralized junction. Type X collagen was in the pericellular matrix of hypertrophic chondrocytes and in some calcified matrix. These data support the theory that the gastrocnemius tendon has fibrocartilage characteristics and that type X collagen has a role in the tissue's mineralization. Anat Rec, 2019. © 2019 Wiley Periodicals, Inc.