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Differential Changes in the Number and Morphology of the New Neurons after Chronic Infusion of Wnt7a, Wnt5a, and Dkk‐1 in the Adult Hippocampus In Vivo
Author(s) -
OrtizMatamoros Abril,
Arias Clorinda
Publication year - 2019
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.24069
Subject(s) - wnt signaling pathway , neurogenesis , neurite , dentate gyrus , neuroscience , stem cell , biology , hippocampus , microbiology and biotechnology , wnt5a , cell fate determination , cellular differentiation , signal transduction , in vitro , genetics , gene , transcription factor
In the adult hippocampus of many mammals, a particular microenvironment in the neurogenic niche regulates the proliferation, self‐renewal, and differentiation of neuronal stem cells. In this proliferative niche, a variety of molecules provide a finely regulated molecular signaling that controls stem cell properties. During development, Wnt signaling has been implicated in cell fate determination and proliferation, in the establishment of cell polarity, as well as a cue for axonal growth and dendrite orientation. In the adult brain, this pathway also participates in the stem cell self‐renewal and neuronal differentiation. However, the effects of the chronic Wnt signaling modulation in the adult hippocampus, through the infusion of Wnt7a, Wnt5a, and Dkk‐1, on the rate of neurogenesis and on the induction of neurite arborization have not been studied. In this study, we show that Wnt7a and Wn5a further increased the rate of newly generated neurons. However, Wnt5a exerted additional effects by promoting neurite growth and neurite misorientation in the dentate gyrus of adult rats. The chronic exposure to Dkk‐1 also generated aberrant location of growing neurites. These results suggest that the interplay of canonical and non‐canonical Wnt ligands participates in neuronal stem cell proliferation and in the establishment of proper neurite maturation. Anat Rec, 302:1647–1657, 2019. © 2019 American Association for Anatomy