Premium
Technical Review: Types of Imaging—Direct STORM
Author(s) -
Jensen Ellen,
Crossman David J.
Publication year - 2014
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.22960
Subject(s) - super resolution microscopy , microscopy , fluorophore , nanotechnology , resolution (logic) , materials science , photoactivated localization microscopy , computer science , optics , physics , artificial intelligence , fluorescence , scanning confocal electron microscopy
In the 1990s, new concepts of microscopy revolutionized the imaging field by breaking the lateral resolution diffraction limit for the first time, even with propagating light and regular lenses (i.e., far‐field). In 2006, several research groups independently showed super‐resolution microscopy using high‐precision localization of single fluorophores. These new developments in single‐molecule spectroscopy enabled a different approach to achieving nanometer‐scale optical microscopy. Direct stochastic optical reconstruction microscopy (dSTORM) is a technique of single‐molecule super‐resolution imaging that does not require an activator fluorophore. This technique is used to visualize cellular structures with a resolution of approximately 20 nm. dSTORM is compatible with many conventionally used fluorophores. This article provides an overview of the principles and uses of dSTORM. Advantages and disadvantages of dSTORM are also discussed. Anat Rec, 297:2227–2231, 2014. © 2014 Wiley Periodicals, Inc.