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The Bacteriocin Sublancin Attenuates Intestinal Injury in Young Mice Infected With Staphylococcus aureus
Author(s) -
Wang Qingwei,
Zeng Xiangfang,
Wang Shuai,
Hou Chengli,
Yang Fengjuan,
Ma Xi,
Thacker Philip,
Qiao Shiyan
Publication year - 2014
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.22941
Subject(s) - staphylococcus aureus , saline , ampicillin , intraperitoneal injection , microbiology and biotechnology , in vivo , bacteriocin , antimicrobial , pharmacology , medicine , biology , antibiotics , bacteria , chemistry , genetics
Sublancin, a bacteriocin, has bactericidal activity against a broad spectrum of gram‐positive bacteria. However, studies have not been conducted to determine its in vivo efficacy against potential pathogens. The objective of this study was to investigate the effects of sublancin in a Staphylococcus aureus ( S. aureus ) infected mouse model which induced intestinal injury. A total of 160, 4‐week‐old mice were randomly assigned to one of eight treatments. Mice in the control group were injected intraperitoneally with 0.5 mL of 0.9% saline. Mice in the other seven groups were given an intraperitoneal injection of 0.5 mL saline containing 1.0 × 10 10 colony‐forming units (CFU)/mL S. aureus . Six hours after inoculation, mice in the control group were again injected with 0.5 mL of 0.9% saline. Mice in the other seven groups were injected intraperitoneally with 0.5 mL of 0.9% saline containing 0, 0.5, 1.0, 2.0, or 4.0 mg/kg body weight (BW) sublancin or 1.0 or 2.0 mg/kg BW ampicillin. The results showed that 4.0 mg/kg sublancin and 2.0 mg/kg ampicillin significantly reduced mice mortality from 55 to 10%. The height and the number of proliferated cells from the intestinal villi in the sublancin and ampicillin treated mice were higher than in the control. We conclude that sublancin has potent antibacterial activity against S. aureus . Therefore, sublancin could find use as an alternative antimicrobial agent for the treatment of gram‐positive bacterial infections. Anat Rec, 297:1454–1461, 2014. © 2014 Wiley Periodicals, Inc.