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Paneth Cell Identification in the Small Intestine of Guinea Pig Offsprings ( Cavia porcellus )
Author(s) -
Vásquez Cachay María Elith,
Gomez Erika Pebe,
Rodríguez Gutiérrez José Luis,
Lira Mejía Boris,
Pérez Néstor Falcón,
Zanuzzi Carolitalia,
Barbeito Claudio
Publication year - 2014
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.22890
Subject(s) - cavia , biology , guinea pig , small intestine , staining , h&e stain , anatomy , immunohistochemistry , eosin , duodenum , tartrazine , pathology , andrology , medicine , endocrinology , immunology , biochemistry , genetics
The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth. Anat Rec, 297:856–863, 2014. © 2014 Wiley Periodicals, Inc.