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Is Mda‐7/IL‐24 a Potential Target and Biomarker for Enhancing Drug Sensitivity in Human Glioma U87 Cell Line?
Author(s) -
Wang Qin,
Zhu Yu,
Yang Ping
Publication year - 2013
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.22723
Subject(s) - glioma , drug resistance , cisplatin , cancer research , efflux , p glycoprotein , cytotoxicity , pharmacology , u87 , cell culture , drug , multiple drug resistance , medicine , biology , chemotherapy , in vitro , microbiology and biotechnology , biochemistry , genetics
Gliomas are the most common form of primary brain tumor with the highest mortality rates. Drug resistance is a major cause of treatment failure in patients with glioma. Melanoma differentiation associated gene‐7/interleukin‐24 (mda‐7/IL‐24) has been demonstrated to play an important role in drug resistance in human cancer cell lines. However, the reversing effect of mda‐7/IL‐24 on drug resistance of human glioma is not fully clear. Here, we investigated the effects of overexpression of the mda‐7/IL‐24 gene in human glioma. We established a cisplatin‐resistant U87 glioma cell line and found that mda‐7/IL‐24 was highly correlated with drug resistance. Furthermore, we investigated the apoptotic rate, intracellular accumulation of Rhodamine‐123, and expression of glutathione and P‐glycoprotein. The over‐expression of mda‐7/IL‐24 enhanced cisplatin cytotoxicity and reversal of drug resistance in glioma cells. The reversing effect of mda‐7/IL‐24 on drug resistance was induced mainly through the regulation of drug resistance‐related genes and efflux drug pumps. Thus, mda‐7/IL‐24 can be used as a promising predictive biomarker and potential therapeutic target for chemotherapy in glioma. Anat Rec, 2013. © 2013 Wiley Periodicals, Inc.