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Growth‐Inhibitory Activity of Melatonin on Murine Foregastric Carcinoma Cells In Vitro and the Underlying Molecular Mechanism
Author(s) -
Xu Li,
Liu Hui,
Zhang Hui,
Wang RiXiong,
Song Jun,
Zhou RuiXiang
Publication year - 2013
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.22689
Subject(s) - downregulation and upregulation , melatonin , in vivo , in vitro , biology , receptor , cell growth , cell culture , cancer research , cell , viability assay , melatonin receptor , pineal gland , apoptosis , microbiology and biotechnology , endocrinology , biochemistry , gene , genetics
Melatonin (MLT) is an indolic hormone produced mainly by the pineal gland. Recent human and animal studies have shown that MLT exerts obvious oncostatic activity both in vitro and in vivo . The purpose of this study was to investigate the antiproliferative effect of MLT on the murine foregastric carcinoma (MFC) cell and to determine the underlying molecular mechanism. Cell viability was determined using the Cell Counting Kit‐8 (CCK‐8) and the results revealed that MLT exhibited a dose‐ and time‐dependent inhibitory effect on MFC cell growth. Our studies also demonstrated upregulation of p21 and Bax and downregulation of Bcl‐2 at both the mRNA and the protein levels in response to MLT treatment of MFC cells. These changes in the expression of these molecules were consistent with the results of the CCK‐8. Furthermore, the mRNA and protein expression of membranous MLT receptors was also upregulated. Taken together, these results confirm the oncostatic effect of MLT in MFC cells and the expression of membranous MLT receptors is a potential approach to tumor cells in gastric cancer therapeutic treatment. Anat Rec, 296:914–920, 2013. © 2013 Wiley Periodicals, Inc.