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Adenovirus‐mediated Interferon‐γ Gene Therapy Induced Human Pancreatic Carcinoma Capan‐2 Cell Apoptosis In Vitro and In Vivo
Author(s) -
Xie FaJun,
Zhao Peng,
Zhang YiPing,
Liu FeiYe,
Nie XiLin,
Zhu YingHui,
Yu XinMin,
Zheng QiuQing,
Mao WeiMin,
Lu HongYang,
Wei Hong,
Huang WenLin
Publication year - 2013
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.22661
Subject(s) - in vivo , genetic enhancement , cancer research , apoptosis , in vitro , pancreatic cancer , interferon , viral vector , adenoviridae , cell , biology , medicine , pathology , gene , immunology , cancer , recombinant dna , biochemistry , genetics , microbiology and biotechnology
Pancreatic cancer is one of the most lethal human malignancies with a very low 5‐year survival rate, which highlights urgent needs for more effective therapeutic strategies. In this study, we examined the potential therapeutic effects of an adenovirus encoding human interferon gamma (Ad‐IFNγ) on pancreatic carcinoma cells Capan‐2 in vitro and in vivo . The results indicated that Ad‐IFNγ could significantly inhibit tumor cell growth via inducing cell apoptosis. After infection, IFNγ expressed durably and stably in xenografts, predominantly in tumor tissue, while much less in blood and liver. Thus, adenovirus‐mediated intratumoral injection of human IFNγ gene could be an effective gene therapeutic system for the treatment of pancreatic carcinoma. Anat Rec, 296:604–610, 2013. © 2013 Wiley Periodicals, Inc.