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Alveolar Epithelial Dynamics in Postpneumonectomy Lung Growth
Author(s) -
Chamoto Kenji,
Gibney Barry C.,
Ackermann Maximilian,
Lee Grace S.,
Konerding Moritz A.,
Tsuda Akira,
Mentzer Steven J.
Publication year - 2013
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.22659
Subject(s) - biology , pneumonectomy , pulmonary alveolus , angiogenesis , alveolar epithelium , alveolar cells , lung , flow cytometry , cell type , immunology , microbiology and biotechnology , cell , pathology , cancer research , medicine , epithelium , respiratory disease , genetics
The intimate anatomic and functional relationship between epithelial cells and endothelial cells within the alveolus suggests the likelihood of a coordinated response during postpneumonectomy lung growth. To define the population dynamics and potential contribution of alveolar epithelial cells to alveolar angiogenesis, we studied alveolar Type II and I cells during the 21 days after pneumonectomy. Alveolar Type II cells were defined and isolated by flow cytometry using a CD45 − , MHC class II + , phosphine + phenotype. These phenotypically defined alveolar Type II cells demonstrated an increase in cell number after pneumonectomy; the increase in cell number preceded the increase in Type I (T1α + ) cells. Using a parabiotic wild type/GFP pneumonectomy model, <3% of the Type II cells and 1% of the Type I cells were positive for GFP—a finding consistent with the absence of a blood‐borne contribution to alveolar epithelial cells. The CD45 − , MHC class II + , phosphine + Type II cells demonstrated the active transcription of angiogenesis‐related genes both before and after pneumonectomy. When the Type II cells on Day 7 after pneumonectomy were compared to nonsurgical controls, 10 genes demonstrated significantly increased expression ( P <0.05). In contrast to the normal adult Type II cells, there was notable expression of inflammation‐associated genes ( Ccl2 , Cxcl2 , Ifng ) as well as genes associated with epithelial growth ( Ereg , Lep ). Together, the data suggest an active contribution of local alveolar Type II cells to alveolar growth. Anat Rec, 296:495–503, 2013. © 2013 Wiley Periodicals, Inc.