Premium
The Expression of Neuronal Nitric Oxide Synthase in the Brain of the Mouse During Embryogenesis
Author(s) -
Ling Shucai,
Zhou Jing,
Rudd John A.,
Hu Zhiying,
Fang Marong
Publication year - 2012
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.22408
Subject(s) - immunohistochemistry , nitric oxide synthase , embryogenesis , neuronal nitric oxide synthase , embryo , biology , hypothalamus , diencephalon , nucleus , inferior colliculus , embryonic stem cell , neuroscience , nitric oxide , microbiology and biotechnology , endocrinology , biochemistry , immunology , gene
The distribution of neuronal nitric oxide synthase (nNOS) in the process of normal mouse brain growth from embryonic (E) Day 11 to postnatal (P) Day 1 was investigated by means of immunohistochemical and immunofluorescent methods. Our results demonstrated that nNOS positive neurons appeared early in superficial cortex at E11. At E13, nNOS positive neurons were located in lateral hypothalamus and amygdala, and temporarily in medullar and ventral hypothalamic neuroepithelia. From E15 to P0, nNOS positive neurons were distributed in superior and inferior colliculi, positive staining could also be seen in superior and inferior tectal neuroepithelium at E15. From E17 to birth, the medial geniculate nucleus had a high density of nNOS labeling. The distribution of nNOS gradually increased and extended laterally in embryo brain, which in turn implies that NO might be involved in the development of mouse brain. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.