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Expression of Mortalin Detected in Human Liver Cancer by Tissue Microarrays
Author(s) -
Chen Xi,
Xu Bo,
Li Hongyan,
Yang Ling,
Zuo Ji,
Liu Wen,
Liu Chenghai
Publication year - 2011
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.21433
Subject(s) - tissue microarray , liver cancer , immunohistochemistry , western blot , carcinogenesis , cancer research , immunocytochemistry , cancer , biology , heat shock protein , dna microarray , pathology , cancer cell , cell culture , hepatocellular carcinoma , cell , gene expression , microbiology and biotechnology , gene , medicine , biochemistry , genetics
Mortalin is a highly conserved molecular chaperone in the heat shock protein (HSP) 70 family, which plays a role in carcinogenesis. The relationship between tumors and the expression of Mortalin is not fully elucidated. In this study, human tumor specimens from various organs of liver cancer at different stages and cultured liver cancer cells were used to study the expression pattern of Mortalin. Through immunohistochemistry we showed that Mortalin was significantly higher in tumors than in adjacent benign tissues. Using liver tissue microarrays tested on hepatocellular carcinomas, Mortalin expression was consecutively higher with advanced tumor stages. Mortalin expression on the cultured liver cancer cells were characterized with immunocytochemistry, Real‐time PCR, and western blot. The results showed that the expression level is markedly higher in the SMMC 7721 (a liver‐derived tumor cell line) than in the HL 7702 (a normal liver cell line) in the protein level only. Understanding the role of Mortalin in tumors may lead to development of a new therapeutic target in cancer treatment. Anat Rec, 2011. © 2011 Wiley‐Liss, Inc.