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ATX–LPA Axis Induces Expression of OPN in Hepatic Cancer Cell SMMC7721
Author(s) -
Zhang Rihua,
Zhang Zhihong,
Pan Xiaolin,
Huang Xiayue,
Huang Zuhu,
Zhang Guoxin
Publication year - 2011
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.21324
Subject(s) - lysophosphatidic acid , autotaxin , cell migration , osteopontin , protein kinase b , pi3k/akt/mtor pathway , chemistry , microbiology and biotechnology , downregulation and upregulation , signal transduction , cancer research , receptor , cell , biology , endocrinology , biochemistry , gene
Osteopontin (OPN) and autotaxin (ATX) are important chemokines involved in the survival, proliferation, migration, invasion, and metastasis of many cancer cells. The focus of the study was to investigate the relationship between OPN and ATX–lysophosphatidic acid (LPA) axis. The expression of OPN and its cellular cascades were determined by western blot and real‐time quantitative transcription polymerase chain reaction (real‐time PCR) analyses. Cell migration activity was determined by a Transwell‐migration assay. In comparison with nontreated cells, we found that the ATX–LPA axis upregulated OPN expression by 2.91‐fold in protein levels and 2.52‐fold in mRNA levels. The ATX–LPA axis activates Akt and ATX/LPC‐induced OPN expression in SMMC7721 cells was largely reduced by the inhibitors of phosphatidylinositol 3‐kinase (PI3K)/Akt or LPA receptor. This study provides the first evidence that the induction of the OPN expression by ATX–LPA axis was mediated by the activation of Akt through LPA receptors and OPN was required for migration of SMMC7721 cells induced by ATX–LPA axis. Anat Rec, 2011. © 2010 Wiley‐Liss, Inc.