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First Evidence of a Leptin‐Like Peptide in a Cartilaginous Fish
Author(s) -
Gambardella Chiara,
Gallus Lorenzo,
Ravera Silvia,
Fasulo Salvatore,
Vacchi Marino,
Ferrando Sara
Publication year - 2010
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.21238
Subject(s) - leptin , immunohistochemistry , gastrointestinal tract , enteroendocrine cell , biology , western blot , stomach , medicine , fish <actinopterygii> , staining , cartilage , endocrinology , scyliorhinus canicula , endocrine system , hormone , pathology , anatomy , fishery , biochemistry , obesity , genetics , gene
Leptin is a hormone involved in food intake. Although leptin is evolutionarily conserved, no studies have investigated its presence in cartilaginous fish. Here, we report the presence of leptin‐like immunoreactivity in the gastro‐intestinal tract and liver of the cartilaginous fish Scyliorhinus canicula using western blot and immunohistochemical analyses. A leptin‐like immunoreactive band of 16 kDa was detected in the homogenate of the stomach, whereas no immunoreactivity was observed in the intestine or the liver. Immunohistochemistry of the gastric mucosa revealed leptin‐like staining localized to mucous‐secreting cells and endocrine cells. This is the first report of a leptin‐like peptide in a cartilaginous fish. Anat Rec 293:1692–1697, 2010. © 2010 Wiley‐Liss, Inc.