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Versican G1 Domain and V3 Isoform Overexpression Results in Increased Chondrogenesis in the Developing Chick Limb In Ovo
Author(s) -
Hudson Karla S.,
Andrews Kristen,
Early June,
Mjaatvedt Corey H.,
Capehart Anthony A.
Publication year - 2010
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.21235
Subject(s) - versican , chondrogenesis , in ovo , microbiology and biotechnology , gene isoform , proteoglycan , extracellular matrix , mesenchyme , anatomy , cartilage , morphogen , biology , glycosaminoglycan , chemistry , mesenchymal stem cell , embryo , biochemistry , gene
Previous work has shown that versican proteoglycan is highly expressed in the extracellular matrix of precartilage limb mesenchyme. Although much of versican's role in chondrogenesis has been attributed to its glycosaminoglycan complement, N‐ and C‐terminal G1 and G3 domains of versican have been shown to possess distinct functions when expressed ectopically. This study was undertaken to test the hypothesis that overexpression of the versican G1 domain and short V3 isoform, comprised of only G1 and G3, in the chick wing in ovo would result in increased chondrogenesis, suggesting function for discrete versican domains in limb skeletal development. Recombinant adenoviruses encoding G1 and V3 proteins were microinjected into proximal HH19–25 chick wing buds which resulted in significant enlargement of humeral primordia at HH35. Enhanced cartilage deposition appeared due to increased chondrogenic aggregation as a result of recombinant G1 or V3 overexpression, further implicating versican in early stages of limb development. Anat Rec 293:1669–1678, 2010. © 2010 Wiley‐Liss, Inc.