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Histochemical and Ultrastructural Observations of Respiratory Epithelium and Gland in Yak ( Bos grunniens )
Author(s) -
Yang Bo,
Yu Sijiu,
Cui Yan,
He Junfeng,
Jin Xinhua,
Wang Ru
Publication year - 2010
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.21056
Subject(s) - serous fluid , lamina propria , epithelium , mucus , biology , ultrastructure , pathology , organelle , staining , cytoplasm , respiratory tract , columnar cell , submucosal glands , mucin , cell type , respiratory epithelium , respiratory system , anatomy , cell , microbiology and biotechnology , biochemistry , medicine , ecology
Abstract Submucous glands and epithelial mucous cells of yak ( Bos grunniens ) respiratory tract have been studied by a variety of histochemical methods and transmission electron microscopy for differentiating and characterizing serous and mucous cells. By light microscopy, the distribution, numbers of mucous cells, volume of mucous glands (Reid index), and the ratio of mucous cell to serous cell in the bronchial tree were measured with different staining. Histochemically, a majority of mucous cells, presented in the surface epithelium of bronchi and glands, secreted neutral and acid mucosubstances, only a few sulfated mucosubstances were present. No mucus‐producing cells were observed from the terminal to respiratory bronchiolar level. Ultrastructurally, serous cells in glands of the lamina propria had two distinct forms: one type filled with many round dense secretory granules, plentiful RER and few other organelles, similar to other animals; the other type contained some oval mitochondrial and distended RER, the granules resembled the former. The mucous cells in gland were similar to that of epithelium, which containing abundant secretory granules with an eccentric core. The mucous cells of the surface epithelium differ from other animals in the structure and histochemistry of their secretory granules. Analysis of the size and distribution of the secretory granules and other organelles of serous cells suggested that differences represent different phases of a secretory cycle, not various populations of cell or granules. Anat Rec 293:1259–1269, 2010. © 2010 Wiley‐Liss, Inc.