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Distribution of Amelotin in Mouse Tooth Development
Author(s) -
Gao Yuguang,
Wang Wanchun,
Sun Yan,
Zhang Juanjuan,
Li Dongliang,
Wei Yahong,
Han Tingting
Publication year - 2010
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.21022
Subject(s) - amelogenin , ameloblast , enamel paint , matrix (chemical analysis) , secretion , microbiology and biotechnology , immunohistochemistry , biology , enamel organ , anatomy , amelogenesis , tooth enamel , dentistry , chemistry , medicine , endocrinology , immunology , chromatography
Amelotin is expressed and secreted by ameloblasts in tooth development, but amelotin distribution during enamel development is not clear. In this report, we first investigated amelotin expression in developing teeth by immunohistochemistry. Amelotin was detected in the enamel matrix at the secretion and maturation stages of enamel development. Amelotin was also observed at Tomes' processes on the apical ends of secretory ameloblasts. We then compared amelotin gene expression with those of amelogenin, enamelin, and ameloblastin in the mandibles of postnatal mice by RT‐PCR. The expression of amelotin was detected as early as in postnatal day 0 mandibles and amelotin was coexpressed with amelogenin, ameloblastin, and enamelin during tooth development. These data strongly suggest that amelotin is an enamel matrix protein expressed at the secretion and maturation stages of enamel development. Anat Rec, 2010. © 2009 Wiley‐Liss, Inc.