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Caveolin‐1 is an Important Factor for the Metastasis and Proliferation of Human Small Cell Lung Cancer NCI‐H446 Cell
Author(s) -
Yeh Dongmei,
Chen Chen,
Sun MingZhong,
Shao Shujuan,
Hao Lihong,
Song Yang,
Gong Linlin,
Hu Jun,
Wang Qi
Publication year - 2009
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.20974
Subject(s) - cell growth , metastasis , biology , transfection , mtt assay , cancer research , cell , cell culture , microbiology and biotechnology , cancer , genetics
Caveolin‐1 (CAV‐1) has been reported to play an important role in the development of a variety of human cancers. CAV‐1 expression is revealed to be reduced or absent in the malignant tumor cells of small cell lung cancers (SCLC). This study was performed to investigate the influences of the stable expression of CAV‐1 on the metastasis and proliferation of SCLC in vitro . The wild‐type CAV‐1 gene was successfully transfected into the NCI‐H446 cells and was stably expressed in the NCI‐H446 cells. The effects of CAV‐1 on the morphology, proliferation, and metastasis potential for NCI‐H446 cell were evaluated by crystal violet staining, MTT analysis, transwell assay, and scratch wound assay, respectively. Western blot and gelatin zymography were used to examine the expression changes of the metastasis‐related MMP‐3 and E‐cadherin. Stable expression of CVA‐1 was observed in the H446‐CAV‐1 cells, which enlarged the cell shape with filopodia. The proliferation of H446‐CAV‐1 was inhibited, while its migration and invasion abilities were promoted in vitro . The re‐expression of CAV‐1 reduced the expression of E‐cadherin, while it increased the protein expression and enzyme activity of MMP‐3. Taken together, the cellular proliferation of the NCI‐H446 could be inhibited by the re‐expression of CAV‐1. CAV‐1 might increase the cell metastasis potential through the interaction with E‐cadherin and MMP‐3 genes. These in vitro findings confirm the involvement of CAV‐1 in the proliferation and metastasis of SCLC. Anat Rec, 2009. © 2009 Wiley‐Liss, Inc.