z-logo
Premium
TGF‐beta1 and TGFBR1 are Expressed in Ameloblasts and Promote MMP20 Expression
Author(s) -
Gao Yuguang,
Li Dongliang,
Han Tingting,
Sun Yan,
Zhang Juanjuan
Publication year - 2009
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.20901
Subject(s) - ameloblast , chemistry , transforming growth factor , microbiology and biotechnology , expression (computer science) , biology , dentistry , medicine , computer science , programming language , enamel paint
Abstract Transforming growth factor‐beta (TGF‐beta) signaling exerts a wide spectrum of biological functions. To investigate TGF‐beta signaling in amelogenesis, we initially assessed the expression of TGF‐beta1 and TGF‐beta receptor 1 (TGFBR1) in developing teeth by immunohistochemistry. Both TGF‐beta1 and TGFBR1 were strongly expressed in secreting ameloblasts. Next, we studied the effects of TGF‐beta signaling on the expression of MMP20 and KLK4 mRNA using ameloblast‐lineage cells (ALC) in vitro. Our RT‐PCR study showed that TGF‐beta1, TGFBR1, and enamel matrix proteases (MMP20 and KLK4) were expressed in ALC. Following TGF‐beta1 treatment, the expression of MMP20 mRNA, but not KLK4 mRNA, was significantly upregulated. To further confirm the TGF‐beta signaling involvement in the MMP20 expression, we constructed the activated TGFBR1 vector and transfected the construct into ALC. The activated TGFBR1 notably promoted MMP20 expression, but had no obvious effects on the KLK4 mRNA expression. Our studies strongly suggest that TGF‐beta signaling involved in amelogenesis is partially mediated by regulating the expression of MMP20 mRNA. Anat Rec, 292:885–890, 2009. © 2009 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here