Premium
Gene Expression Profiling in TWIST‐Depleted Gastric Cancer Cells
Author(s) -
Feng MeiYan,
Wang Kuan,
Shi QingTao,
YU XiuWen,
Geng JingShu
Publication year - 2009
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.20802
Subject(s) - epithelial–mesenchymal transition , cancer research , microbiology and biotechnology , biology , twist transcription factor , transcription factor , cancer cell , cancer , gene expression profiling , metastasis , small interfering rna , myb , suppressor , gene , gene expression , rna , genetics
TWIST is an important transcription factor during embryonic development and has recently been found to promote the epithelial‐mesenchymal transition (EMT) phenomenon seen during the initial steps of tumor metastasis. To further investigate the potential targets and interacting genes of TWIST in human gastric cancer, we performed microarray analysis to compare the gene expression profiles in HGC‐27 cells, with or without small interfering RNA (siRNA)‐mediated depletion of TWIST. Our results showed that NF1 , RAP1A , SRPX , RBL2 , PFDN4 , ILK , F2R , ERBB3 , and MYB were up‐regulated, whereas AKR1C2 , FOS , GDF15 , NR2F1 , ATM , and CTPS were down‐regulated after TWIST depletion. Moreover, TWIST‐depleted HGC‐27 cells showed a reversal of the morphologic and molecular changes associated with EMT. These results provide evidence that TWIST regulates the expression of several genes involved in the differentiation, adhesion, and proliferation of gastric cancer cells. The role of TWIST in the development of certain types of gastric cancer is discussed. Anat Rec, 2009. © 2008 Wiley‐Liss, Inc.