Premium
Protective Effect of Glutathione Against Liver Warm Ischemia‐Reperfusion Injury in Rats is Associated with Regulation of P‐Selectin and Neutrophil Infiltration
Author(s) -
Xue Fei,
Wang Guangtian,
Pang Zhigang,
Liu Chao,
Liang Tingbo
Publication year - 2008
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.20725
Subject(s) - glutathione , infiltration (hvac) , liver injury , ischemia , liver transplantation , reperfusion injury , medicine , hepatocyte , p selectin , liver function , portal vein , endocrinology , pharmacology , immunology , pathology , transplantation , chemistry , in vitro , biochemistry , platelet , enzyme , platelet activation , physics , thermodynamics
Abstract We examined the effects of glutathione (GSH) preconditioning through the portal vein on rat warm liver ischemia reperfusion injury (I/R injury) and investigated the mechanisms involved. In rats with warm liver I/R injury, administration of GSH by means of the portal vein before ischemia increased the 7‐day survival rates of rats after liver I/R from 38% to 75%. This effect was correlated with significantly improved liver function, depressed MDA content in the liver and fewer histologic features of hepatocyte injury. Intrahepatic expression of P‐selectin and infiltration of neutrophils were increased significantly after liver I/R. GSH pretreatment decreased intrahepatic MPO content and the expression of P‐selectin. However, it did not significantly affect the mRNA levels for P‐selectin after liver I/R. Thus, preconditioning with GSH protects the liver against I/R injury by a mechanism dependent on free radical species scavenging, down‐regulation of adhesion molecule expression and inhibition of neutrophil accumulation. These findings document the potential clinical utility of GSH to improve the overall success of diverse procedures, such as liver surgery and liver transplantation. Anat Rec, 291:1016–1022, 2008. © 2008 Wiley‐Liss, Inc.