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pCREB is Involved in Neural Induction of Mouse Embryonic Stem Cells by RA
Author(s) -
Shan ZhiYan,
Shen JingLing,
Li QiuMing,
Wang Yan,
Huang XiaoYi,
Guo TieYun,
Liu HuiWen,
Lei Lei,
Jin LianHong
Publication year - 2008
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.20686
Subject(s) - creb , embryoid body , retinoic acid , embryonic stem cell , microbiology and biotechnology , progenitor cell , neural stem cell , cellular differentiation , biology , stem cell , chemistry , transcription factor , cell culture , adult stem cell , biochemistry , genetics , gene
Mouse embryonic stem (ES) cells can be induced by various chemicals to differentiate into a variety of cell types in vitro. In our study, retinoic acid (RA), one of the most important inducers, used at a concentration of 5 μM, was found to induce the differentiation of ES cells into neural progenitor cells (NPCs). During embryoid body (EB) differentiation, the level of active cyclic AMP response element‐binding protein (CREB) was relatively high when 5 μM RA treatment was performed. Inhibition of CREB activity committed EBs to becoming other germ layers, whereas increased expression of CREB enhanced NPC differentiation. Moreover, RA increased the expression of active CREB by enhancing the activity of JNK. Our research suggests that CREB plays a role in RA‐induced NPC differentiation by increasing the expression of active JNK. Anat Rec, 291:519–526, 2008. © 2008 Wiley‐Liss, Inc.