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Ultrastructural comparison of the morphology of three different platelet and fibrin fiber preparations
Author(s) -
Pretorius Etheresia,
Briedenhann Sharon,
Marx Jorika,
Smit Eureka,
Van Der Merwe Chris,
Pieters Marlien,
Franz Carl
Publication year - 2007
Publication title -
the anatomical record: advances in integrative anatomy and evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.678
H-Index - 62
eISSN - 1932-8494
pISSN - 1932-8486
DOI - 10.1002/ar.20413
Subject(s) - fibrin , ultrastructure , fibrinogen , platelet , scanning electron microscope , morphology (biology) , chemistry , electron microscope , biophysics , hemostasis , pathology , biomedical engineering , biology , materials science , biochemistry , composite material , immunology , medicine , genetics , physics , optics
The aim of the current study was to investigate the ultrastructural morphology of three different sources of fibrin networks and platelets, namely, lypholized human platelet‐rich plasma (LPRP), freshly prepared human platelet‐rich plasma (FPRP), and human platelet concentrate (HPC). The ultrastructural morphology of the three different fibrin networks was studied using the scanning electron microscope (SEM). Turbidity curves were drawn at 405 nm at room temperature and fibrinogen concentrations were measured. Scanning electron micrographs showed that all clots produced thick major fibrin fibers as well as a well‐defined fine fibrin network, which appeared to be a superimposed process that occurred after the major fibrin network was established. These features were decidedly more pronounced in the HPC specimens. Turbidity curves of the three types of plasma showed differences in LPRP and FPRP. Fibrinogen concentrations of all three preparations were in the normal ranges. Because of the great similarity between LPRP, HPC, and FPRP, we suggest that LPRP could be used successfully to study morphological changes in fibrin fibers and platelets, which may occur after exposure to certain therapeutic agents. However, functionality studies such as turbidity curves should concurrently be included. We therefore conclude that from a basic science point of view, LPRP is a valuable research tool and that such results may add information that could be valuable for clinical application. Anat Rec 290:188–198, 2007. © 2007 Wiley‐Liss, Inc.