Open AccessHuman reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors
Author(s) -
Young Henry E.,
Steele Timothy A.,
Bray Robert A.,
Hudson John,
Floyd Julie A.,
Hawkins Kristina,
Thomas Karen,
Austin Troy,
Edwards Chris,
Cuzzourt Jeremy,
Duenzl Mary,
Lucas Paul A.,
Black Asa C.
Publication year - 2001
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
eISSN - 1097-0185
pISSN - 0003-276X
DOI - 10.1002/ar.1128
Subject(s) - stem cell , biology , induced pluripotent stem cell , mesenchymal stem cell , stem cell transplantation for articular cartilage repair , adult stem cell , amniotic stem cells , cd90 , cd34 , microbiology and biotechnology , endothelial stem cell , pathology , embryonic stem cell , medicine , biochemistry , in vitro , gene
Abstract This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity of the stem cells from each population. All populations contained lineage‐committed myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as lineage‐uncommitted pluripotent stem cells capable of forming muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells. Flow cytometric analysis of adult stem cell populations revealed positive staining for CD34 and CD90 and negative staining for CD3, CD4, CD8, CD11c, CD33, CD36, CD38, CD45, CD117, Glycophorin‐A, and HLA DR‐II. Anat Rec 264:51–62, 2001. © 2001 Wiley‐Liss, Inc.