A multiplex microsatellite tool for conservation genetics of the endemic limpet Patella candei in the Macaronesian archipelagos

The intertidal limpet Patella candei represents an important exploited resource in the Macaronesian archipelagos of Azores, Madeira and Canaries (NE Atlantic). Despite being considered endemic, the taxonomic status of P . candei throughout the region remains unclear. The aim of this study was to develop novel microsatellite markers using next generation sequencing for the limpet P . candei . Twelve novel loci were described and amplified in 103 individuals from two populations (Azores and Madeira) using three multiplex reactions. The number of alleles per locus ranged from 2 to 14 and global observed heterozygosity was 0.417. Genetic differentiation between samples was highly significant ( F ST  = 0.427) as two main gene pools ( K  = 2) were identified using Bayesian approaches. The present genetic tool can be useful to establish the genetic structuring and patterns of connectivity of P . candei in Macaronesia as well as to determine the number of extant subspecies within the P . candei species complex. Such data will provide a fundamental asset to define stocks and thus inform specific conservation strategies that foster the sustainable exploitation of the P . candei complex throughout Macaronesia archipelagos. Copyright © 2016 John Wiley & Sons, Ltd.