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A multiplex microsatellite tool for conservation genetics of the endemic limpet Patella candei in the Macaronesian archipelagos
Author(s) -
Faria João,
Pita Alfonso,
Rivas Manuel,
Martins Gustavo M.,
Hawkins Stephen J.,
Ribeiro Pedro,
Neto Ana I.,
Presa Pablo
Publication year - 2016
Publication title -
aquatic conservation: marine and freshwater ecosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 77
eISSN - 1099-0755
pISSN - 1052-7613
DOI - 10.1002/aqc.2651
Subject(s) - biology , limpet , archipelago , subspecies , microsatellite , conservation genetics , endemism , evolutionary biology , ecology , allele , genetics , intertidal zone , gene
The intertidal limpet Patella candei represents an important exploited resource in the Macaronesian archipelagos of Azores, Madeira and Canaries (NE Atlantic). Despite being considered endemic, the taxonomic status of P . candei throughout the region remains unclear. The aim of this study was to develop novel microsatellite markers using next generation sequencing for the limpet P . candei . Twelve novel loci were described and amplified in 103 individuals from two populations (Azores and Madeira) using three multiplex reactions. The number of alleles per locus ranged from 2 to 14 and global observed heterozygosity was 0.417. Genetic differentiation between samples was highly significant ( F ST = 0.427) as two main gene pools ( K = 2) were identified using Bayesian approaches. The present genetic tool can be useful to establish the genetic structuring and patterns of connectivity of P . candei in Macaronesia as well as to determine the number of extant subspecies within the P . candei species complex. Such data will provide a fundamental asset to define stocks and thus inform specific conservation strategies that foster the sustainable exploitation of the P . candei complex throughout Macaronesia archipelagos. Copyright © 2016 John Wiley & Sons, Ltd.