Open AccessIsolation and identification of EST ‐ SSR markers in Ixonanthes chinensis (Ixonanthaceae)
Author(s) -
Guo Wei,
Fan Qiang,
Wang Jinjiang,
Meng Kaikai,
Chen Sufang,
Zhu Liping,
Liao Wenbo
Publication year - 2019
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1246
Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , genetics , contig , primer (cosmetics) , genetic marker , allele , expressed sequence tag , gene , genome , chemistry , organic chemistry
Premise Ixonanthes (Ixonanthaceae) consists of between three and 19 species, among which I. chinensis and I. khasiana are considered vulnerable. Here, 58 microsatellite markers were developed for further conservation of these two Ixonanthes species. Methods and Results RNA transcripts of I. chinensis were sequenced and assembled into 19,545 unigenes, and 994 simple sequence repeat ( SSR ) loci were identified from 920 contigs. Based on these, 106 primer pairs were designed, 58 were successfully amplified, and 12 demonstrated polymorphism among five populations. The number of alleles per locus varied from three to 10, and the levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and 0.000 to 0.844, respectively. Further assessment of the transferability of the 58 amplifiable primers reported 30 being successfully cross‐amplified in I. icosandra and three in Erythroxylum sinense . Conclusions These novel SSR markers will be useful for future genetic conservation studies on these Ixonanthes species.