Open AccessMicrosatellite primer development for the invasive perennial herb Gypsophila paniculata (Caryophyllaceae)
Author(s) -
LeimbachMaus Hailee B.,
Parks Syndell R.,
Partridge Charlyn G.
Publication year - 2018
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1203
Subject(s) - biology , microsatellite , caryophyllaceae , genetic diversity , genetic structure , genetics , botany , genetic variation , population , allele , gene , demography , sociology
Premise of the Study Gypsophila paniculata (baby's breath; Caryophyllaceae) is a herbaceous perennial that has invaded much of northern and western United States and Canada, outcompeting and crowding out native and endemic species. Microsatellite primers were developed to analyze the genetic structure of invasive populations. Methods and Results We identified 16 polymorphic nuclear microsatellite loci for G. paniculata out of 73 loci that successfully amplified from a primer library created using Illumina sequencing technology. Microsatellite primers were developed to amplify di‐, tri‐, and tetranucleotide repeats and tested in three invasive populations in Michigan. Conclusions These markers will be useful in characterizing the genetic structure of invasive populations throughout North America to aid targeted management efforts, and in native Eurasian populations to better understand invasion history. Five of these developed primers also amplified in G. elegans .