Open AccessMicrosatellite markers for the prized matsutake mushroom ( Tricholoma matsutake , Tricholomataceae)
Author(s) -
Li JianWen,
Yin Xin,
Zhao YouJie,
Yang ShuJiao,
Vaario LuMin,
Yang XueFei
Publication year - 2018
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1202
Subject(s) - biology , mushroom , microsatellite , botany , zoology , genetics , gene , allele
Premise of the Study Novel and cost‐effective microsatellite markers were developed to explore the population genetics, biogeographic structure, and evolutionary history of the prized Euro‐Asian wild edible ectomycorrhizal fungus Tricholoma matsutake (Tricholomataceae). Methods and results Eighteen new polymorphic simple sequence repeat loci, detected from a microsatellite‐enriched genomic library, were used to characterize 131 individuals from eight T. matsutake populations. The number of alleles ranged from two to 10, with averages of 1.42 to 3.22. Levels of observed and expected heterozygosity ranged from 0.00–1.00 and from 0.00–0.83, with mean values of 0.21 and 0.26, respectively. In total, 50% of the loci showed interspecific transferability and polymorphism in the related species T. equestre . Conclusions These newly developed markers will aid research into the genetic diversity and population structure of T. matsutake . They can also be used in other species of Tricholoma .