Open AccessMicrosatellite markers for Corybas (Orchidaceae) species in New Zealand
Author(s) -
Van Etten Megan L.,
Lehnebach Carlos A.,
Pearson Sofie M.,
Robertson Alastair W.,
Tate Jennifer A.
Publication year - 2018
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1192
Subject(s) - biology , microsatellite , orchidaceae , genetic diversity , locus (genetics) , taxon , population , population genetics , evolutionary biology , allele , zoology , ecology , genetics , gene , demography , sociology
Premise of the Study Microsatellite markers were developed for New Zealand species of Corybas (Orchidaceae) to investigate population genetics and species delimitation. Methods and Results From sequencing a total genomic DNA library (using Illumina MiSeq), we developed 22 microsatellite markers for C. obscurus . The di‐ and trinucleotide repeat loci were initially trialed on individuals representing seven Corybas taxa ( C . “rimutaka,” C. confusus , C. hypogaeus , C. macranthus , C. obscurus , C. trilobus , and C. walliae ) and had one to eight alleles per locus. Twelve polymorphic markers were further tested on six Corybas populations from three of the seven taxa ( C. obscurus , C . “rimutaka,” and C. trilobus ). Observed and expected heterozygosities ranged from 0–1 and 0–0.859, respectively. The utility of these 12 loci was further validated in five related Corybas species ( C. hypogaeus , C. obscurus , C. vitreus , C. walliae , and C . “rimutaka”; 38 individuals) representing populations from across the North and South Islands. The average value for genetic diversity among populations ( F ST ) of 0.439 shows differentiation among species. Conclusions These markers will be useful for future studies aimed at delimiting species boundaries and examining the genetic diversity of the New Zealand Corybas species.