Open AccessSSR markers for Filago subg. Filago (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgenera
Author(s) -
GutiérrezLarruscain David,
Malvar Ferreras Teresa,
MartínezOrtega M. Montserrat,
Rico Enrique,
AndrésSánchez Santiago
Publication year - 2018
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1171
Subject(s) - biology , microsatellite , subgenus , locus (genetics) , phylogenetic tree , asteraceae , genetics , population , evolutionary biology , genus , allele , botany , gene , demography , sociology
Premise of the Study Microsatellite primers were developed for the first time in the genus Filago (Gnaphalieae: Asteraceae). These markers will facilitate low‐scale phylogenetic, phylogeographic, and population genetic studies within the genus Filago . Methods and Results Ten pairs of polymorphic microsatellite primers (as well as five pairs of monomorphic primers) were identified and optimized on two species of Filago ( F. gaditana and F. carpetana ) using a microsatellite‐enrichment library method and 454 GS ‐ FLX technique. The polymorphic primers amplified tri‐ to hexanucleotide repeats and showed one to six alleles per locus for both species. Transferability was performed in 29 samples corresponding to nine representative species of Filago . Conclusions The results indicate the utility of the newly developed markers, which will be useful to delve into the phylogenetic relationships among the taxa within Filago . These microsatellites will enable studies of phylogeographic, reproductive, and genetic variation.