Open AccessIsolation and characterization of microsatellite loci for Prunus mongolica (Rosaceae)
Author(s) -
Cheng YuChen,
Zhang DeJian,
Lu ZhanYuan,
Ye XueSong,
Wang JianGuo,
Sun Ping,
Zhang BaoWei
Publication year - 2018
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1158
Subject(s) - biology , microsatellite , genetic diversity , phylogeography , loss of heterozygosity , genetic structure , population , prunus , evolutionary biology , rosaceae , genetics , allele , botany , genetic variation , phylogenetic tree , gene , demography , sociology
Premise of the Study Microsatellite primers were developed in Prunus mongolica (Rosaceae), a relict flora endemic in arid areas of the Asian interior, to investigate the genetic diversity, phylogeography, population structure, and history of the species. Methods and Results Fifty‐one microsatellite loci, including di‐, tri‐, and tetranucelotide repeats, were identified using transcriptome sequencing and bioinformatic screening. The number of alleles ranged from seven to 11 and the levels of observed and expected heterozygosity ranged from 0.545 to 1.000 and 0.600 to 0.989, respectively. Most of the primers also amplified in a group of congeneric species ( P. triloba , P. davidiana , P. persica , P. cerasifera , and P. serrulata ). Conclusions This set of microsatellite loci is useful for studying the genetic diversity of P. mongolica . In addition, they can also be used to investigate the population structure, phylogeography, and landscape genetic patterns of congeneric species.