Open AccessMicrosatellite marker development for the tetraploid Veronica aragonensis (Plantaginaceae) using next‐generation sequencing and high‐resolution melting analyses
Author(s) -
PadillaGarcía Nélida,
MalvarFerreras Teresa,
Lambourdière Josie,
MartínezOrtega M. Montserrat,
Machon Nathalie
Publication year - 2018
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1154
Subject(s) - biology , microsatellite , subspecies , high resolution melt , plantaginaceae , genetic diversity , population , loss of heterozygosity , locus (genetics) , genetics , evolutionary biology , allele , ion semiconductor sequencing , genetic marker , dna sequencing , zoology , genotype , botany , gene , demography , sociology
Premise of the Study The tetraploid Veronica aragonensis (Plantaginaceae) is a narrow endemic to the Iberian Peninsula. Specific microsatellite markers were developed to investigate genetic structure and diversity. Methods and Results A total of 15 polymorphic markers were characterized on three populations of V. aragonensis , using a microsatellite‐enriched library on an Ion Torrent sequencer and high‐resolution melting ( HRM ) analyses to rapidly discard nonreliable, multicopy, and/or monomorphic loci. Allele number per locus ranged from one to five, and levels of observed heterozygosity per population varied from 0.142 ± 0.301 to 0.281 ± 0.369. Most primers also amplified in the closely related species V. rosea and in three subspecies of V. tenuifolia . Conclusions The species‐specific microsatellite markers developed here represent an essential tool to provide genetic information on the population level for V. aragonensis . The low levels of variation detected highlight the importance of continued efforts to improve conservation of the species.