Open AccessDevelopment and characterization of 23 polymorphic microsatellite loci for Amentotaxus argotaenia (Taxaceae), a relict vulnerable species
Author(s) -
Huang Qiqi,
Wang Zhen,
Wang Ting,
Su Yingjuan
Publication year - 2018
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1149
Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , amplified fragment length polymorphism , allele , population , genetics , genetic structure , genetic variation , genetic marker , genetic diversity , population genetics , evolutionary biology , gene , demography , sociology
Premise of the Study New microsatellite markers were developed for the vulnerable conifer species Amentotaxus argotaenia (Taxaceae) to investigate population genetic variation and the effects of environmental heterogeneity on genetic structure. Methods and Results A total of 27 microsatellite loci were developed from A. argotaenia through a Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, of which 23 were polymorphic. These markers yielded 1–13 alleles and 1.0–7.9 effective alleles per locus; levels of observed and expected heterozygosity varied from 0.000–1.000 and 0.000–0.873, respectively. In total, 18 of the markers were transferable to the related species A. yunnanensis . Conclusions These polymorphic markers are a valuable genetic resource for investigating population genetic variation and the potential for local adaptation in A. argotaenia .