Open AccessDevelopment of 26 microsatellite markers in Bupleurum latissimum (Apiaceae), an endangered plant endemic to Ulleung Island, Korea
Author(s) -
Lee JungHyun,
Yoon Chang Young,
Han EunKyeong,
Kim JooHwan
Publication year - 2018
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.1144
Subject(s) - biology , endangered species , apiaceae , microsatellite , loss of heterozygosity , evolutionary biology , endemism , allele , conservation genetics , genetics , botany , ecology , gene , habitat
Premise of the Study To enhance our understanding of evolutionary consequences and to establish a suitable conservation strategy, we isolated microsatellite markers for the endangered Bupleurum latissimum (Apiaceae), which is endemic to the oceanic Ulleung Island. We also attempted cross‐amplification in B. euphorbioides and B. longeradiatum to investigate its continental progenitors. Methods and Results Using high‐throughput sequencing data, we developed 26 polymorphic microsatellite loci in three multiplexes, of which 13 loci were polymorphic in the two related species. For B. latissimum , alleles numbered two to four and the observed and expected heterozygosity ranged from 0.000 to 0.500 and 0.061 to 0.529, respectively. Conclusions These developed markers will be useful for understanding evolutionary patterns of B. latissimum in an oceanic island system and for establishing suitable conservation strategies at the genetic level.