Open AccessDevelopment of chloroplast microsatellite markers for giant ragweed ( Ambrosia trifida )
Author(s) -
Sharma Himanshu,
Hyvönen Jaakko,
Poczai Péter
Publication year - 2020
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.11313
Subject(s) - biology , microsatellite , genetic diversity , locus (genetics) , chloroplast dna , noxious weed , ambrosia artemisiifolia , loss of heterozygosity , botany , allele , weed , genetics , evolutionary biology , chloroplast , ragweed , population , gene , immunology , allergy , demography , sociology
Premise Plant invasions are increasing globally, and extensive study of the genetic background of the source and invading populations is needed to understand such biological processes. For this reason, chloroplast microsatellite markers were identified to explore the genetic diversity of the noxious weed Ambrosia trifida (Asteraceae). Methods and Results The complete chloroplast genome of A. trifida was mined for microsatellite loci, and 15 novel chloroplast primers were identified to assess the genetic diversity of 49 Ambrosia samples. The number of alleles amplified ranged from two to six, with an average of 3.2 alleles per locus. Shannon's information index varied from 0.305 and 1.467, expected heterozygosity ranged from 0.178 to 0.645, and the polymorphism information content value ranged from 0.211 to 0.675 (average 0.428). The cross‐species transferability of the 15 microsatellite loci was also evaluated in four related Ambrosia species ( A. artemisiifolia , A. maritima , A. psilostachya , and A. tenuifolia ). Conclusions The novel chloroplast microsatellite markers developed in the current study demonstrate substantial cross‐species transferability and will be helpful in future genetic diversity studies of A. trifida and related species.