Open AccessDevelopment and characterization of microsatellite markers in Campomanesia adamantium , a native plant of the Cerrado ecoregions of South America
Author(s) -
Crispim Bruno do Amaral,
Déo Thamiris Gatti,
Fernandes Juliana dos Santos,
Vasconcelos Adrielle Ayumi,
Vieira Maria do Carmo,
Carnevali Thiago de Oliveira,
Bajay Miklos Maximiliano,
Zucchi Maria Imaculada,
Barufatti Alexeia
Publication year - 2019
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.1002/aps3.11287
Subject(s) - microsatellite , biology , locus (genetics) , genetic diversity , allele , population , genetic marker , evolutionary biology , genetics , primer (cosmetics) , botany , gene , demography , sociology , chemistry , organic chemistry
Premise A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora . Methods and Results Forty‐one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross‐amplification was tested in C. sessiliflora ; more than 55.5% of the microsatellite loci amplified, confirming the use of these microsatellite markers in a related species. Conclusions We developed a set of microsatellite markers that will be useful for future studies of genetic diversity and population structure of C. adamantium and a closely related species, which will aid in future conservation efforts.