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Construction of gold nanoparticles by tubular polysaccharide from black fungus and their apoptosis‐inducing activities in HepG2 cells
Author(s) -
Song Haoying,
Hu Na,
Gao Ziwei,
Zhang Baohui,
Hu Junjie,
Qiu Zhenpeng,
Zheng Guohua,
Chang Cong,
Meng Yan
Publication year - 2021
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.51537
Subject(s) - polysaccharide , apoptosis , colloidal gold , nanoparticle , substrate (aquarium) , fungus , helix (gastropod) , mitochondrion , biophysics , in vitro , chemistry , materials science , nanotechnology , microbiology and biotechnology , biochemistry , biology , ecology , botany , snail
Gold nanoparticles (AuNPs) with size of 12 ~ 25 nm were loaded on the dendritic nanotubes (DNTs), which were self‐assembled by the triple‐helix polysaccharides from black fungus (AF1). The characterization results proved that the AuNPs were dispersed on the surface of DNTs without affecting their tubular structure. Due to the dendritic structure, the loading content of AuNPs could arrived at 46.4%. Moreover, the potential anticancer activities of the complex (DNT‐Au) were evaluated by the induction of apoptosis in HepG2 cells. It was found DNT‐Au could be taken up by cells and enter into lysosomes, further inducing apoptosis in HepG2 cells by ROS‐mediated mitochondrial dysfunction. This work was benefit for the development of natural polysaccharide as a substrate to stabilize nanoparticles in biomedical fields.