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Immobilization of multicopper oxidase from Pyrobaculum aerophilum onto an electrospun‐aligned single‐walled carbon nanotube surface via a carbon‐nanotube‐binding peptide for biocathode
Author(s) -
Sakamoto Hiroaki,
Futamura Rie,
Fujiwara Ikuya,
Meboso Taichi,
Li Ning,
Takamura Eiichiro,
Satomura Takenori,
Suye Shinichiro
Publication year - 2021
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.50937
Subject(s) - carbon nanotube , nanotechnology , materials science , nanotube , electrode , specific surface area , glucose oxidase , surface modification , biosensor , chemistry , catalysis , biochemistry
Biofuel cells (BFCs) that produce electrical energy from organic resources through enzymatic reactions have been attracting significant attention. Owing to the high electrical conductivity of carbon nanotubes (CNTs), their modification on the electrode surface of a BFC is expected to increase the current, and their high specific surface area may be useful in increasing the power output. Previously, we constructed a biocathode by immobilizing multicopper oxidase from Pyrobaculum aerophilum (McoP) with a carbon nanotube binding peptide (CBP) sequence on the CNTs. This resulted in higher current densities than when using enzymes without CBP sequences. However, owing to the randomly stacked CNTs on the surface of the electrodes, their conductive properties were impaired and performance as biocathodes was poor. Herein, we constructed a biocathode in which single‐walled CNTs (SWCNTs) were oriented one‐dimensionally and McoP is immobilized on the surface of an SWNCT via CBP. The current density was successfully increased by two‐fold by orienting the CNTs and orienting and immobilizing McoP on their surfaces. This technology provides insights into the development of biodevices with controlled orientation of both the SWCNTs and enzymes immobilized on their surfaces.