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Preparation of zinc‐coordinated‐DPA functionalized polyesters for gene condensation
Author(s) -
Shao Xuefei,
He Junnan,
Zhao Xuemei,
Zheng Yubin,
Zheng Nan
Publication year - 2021
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.50843
Subject(s) - polyester , polymer , amine gas treating , cationic polymerization , zinc , condensation polymer , gene delivery , surface modification , condensation , zinc nitrate , combinatorial chemistry , chemistry , dna condensation , materials science , polymer chemistry , organic chemistry , gene , genetic enhancement , biochemistry , transfection , physics , thermodynamics
Abstract Cationic polyesters have been widely utilized as efficient gene delivery carriers. Their ability in binding genes was majorly based on the electrostatic effect between the positive charges of polymers and the negatives charges of genes. It has been well known that large numbers of positive charges on the polymers would lead to undesired toxicity although strong gene binding capability. It was of great interest to developed a polymer with reduced positive charges while enhanced gene condensation ability. In this work, a library of polyesters functionalized by zinc‐coordinated‐dimethylpyridinium amine (DPA‐Zn) have been successfully prepared by the polycondensation method starting from dimethyl 1,3‐acetonedicarboxylate and 10 diols, followed by the post‐modification using dimethylpyridinium amine and zinc nitrate. The post‐modification efficiency was systemically evaluated and the optimized functionalization efficiency could reach around 50%. The gene condensation ability of the targeting polymers was also evaluated using gel retardation assay and dynamic light scatting. The results indicated that DPA‐Zn functionalized polyesters could bind gene into nanocomplexes with the sizes around 200 nm.