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Cover Image, Volume 138, Issue 22
Author(s) -
Erfani Amir,
Zarrintaj Payam,
Seaberg Joshua,
Ramsey Joshua D.,
Aichele Clint P.
Publication year - 2021
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.50699
Subject(s) - covalent bond , immobilized enzyme , polymer chemistry , emulsion polymerization , methacrylate , chemistry , reusability , methyl methacrylate , biocompatible material , polymerization , chemical engineering , materials science , radical polymerization , emulsion , enzyme , organic chemistry , polymer , computer science , engineering , medicine , software , programming language , biomedical engineering
There is emerging evidence showing how biocompatible zwitterionic materials can prevent non‐specific interactions within protein systems, increasing protein stability. In this cover designed by Clint P. Aichele and collagues, a zwitterionic microgel was synthesized from poly (carboxybetaine methyl methacrylate) (pCB) using an inverse emulsion, a free radical polymerization reaction technique. The microgel was loaded with a model enzyme, α‐chymotrypsin (ChT), using a post‐fabrication loading technique. A reaction scheme was developed and studied for covalent immobilization of ChT within the microgel. The enzyme‐immobilized microgels showed excellent reusability and increased enzymatic stability. Furthermore, enzyme half‐life increased up to seven‐fold for the pCB immobilized ChT, and the increased stability resulted in higher activity at elevated pH. Doi: 10.1002/app.50545