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Spectrally encoded microspheres for immunofluorescence analysis
Author(s) -
Sankova Natalya,
Shalaev Pavel,
Semeykina Viktoriya,
Dolgushin Sergey,
Odintsova Elena,
Parkhomchuk Ekaterina
Publication year - 2021
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.49890
Subject(s) - microsphere , immunofluorescence , multiplex , materials science , nanotechnology , microparticle , fluorescence , optics , chemical engineering , physics , engineering , antibody , bioinformatics , medicine , biology , immunology
A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere‐based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere‐based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead‐based immunofluorescence analysis.

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