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Electrospun nanofibrous alginate sulfate scaffolds promote mesenchymal stem cells differentiation to chondrocytes
Author(s) -
Irani Shiva,
Tavakkoli Sajjad,
PezeshkiModaress Mohamad,
Taghavifar Elham,
Mohammadali Marjan,
Daemi Hamed
Publication year - 2021
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.49868
Subject(s) - chondrogenesis , polyvinyl alcohol , mesenchymal stem cell , nanofiber , electrospinning , aggrecan , mtt assay , chemistry , chondrocyte , cartilage , tissue engineering , biomedical engineering , materials science , cell , microbiology and biotechnology , nanotechnology , polymer , anatomy , biochemistry , pathology , medicine , biology , articular cartilage , osteoarthritis , organic chemistry , alternative medicine
Cartilage tissue engineering is one of the interesting approaches used for repairing cartilage injuries. This study reports the fabrication of polyvinyl alcohol/alginate sulfate (PVA/ALG‐S) nanofibrous mats as a functional support for chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBM‐MSCs). The PVA/ALG‐S nanofibers were obtained through electrospinning of PVA solutions containing 10, 20, and 30 wt% of ALG‐S. The appearance of a band at 833 cm −1 assigned to the symmetrical COS vibration associated to a COSO 3 group confirmed the presence of ALG‐S in nanofibrous mat. The SEM images illustrated the bead‐free and smooth morphology of PVA/ALG‐S nanofibers with a mean diameter of 185 ± 0.06 nm. The MTT assay of the hBM‐MSCs seeded on scaffolds indicated the appropriate cytocompatibility of nanofibrous PVA/ALG‐S scaffolds. Furthermore, the appropriate attachment and spreading of the hBM‐MSCs based on SEM images, and their differentiation to the chondrocyte‐like cells accompanied by a decrease in cell growth on MTT analysis and more color absorption in alician blue staining indicated the effective role of alginate sulfate on cell differentiation. Finally, the expression of Type II collagen by RT‐PCR and immunocytochemistry analyses revealed the chondrogenic differentiation of hBM‐MSCs on alginate sulfate nanofibers.