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Layer‐by‐layer self‐assembly of collagen and chitosan biomolecules on polyurethane films
Author(s) -
Bahrami Narges,
Nouri Khorasani Saied,
Mahdavi Hamid,
Khalili Shahla
Publication year - 2020
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.49417
Subject(s) - biocompatibility , chitosan , polyurethane , surface modification , materials science , hexamethylene diisocyanate , layer by layer , polymer chemistry , chemical engineering , nuclear chemistry , layer (electronics) , nanotechnology , chemistry , composite material , engineering , metallurgy
Polyurethane (PU) films used in wound dressing applications often have appropriate properties. Still, surface modification is necessary to increase the biocompatibility. In this research, the surface of the PU films was modified with collagen and chitosan biomolecules through layer by layer (LbL) self‐assembly process. The PU films were synthesized from castor oil and hexamethylene diisocyanate. Then, they were treated with low‐pressure nitrogen plasma to graft with poly(acrylic acid). Before performing the LbL process, the surfaces of the PU films were modified using three different reagents, including (A) NaOH solution, (B) EDC/NHS solution, or (C) hexamethylene diamine. Then, the collagen and chitosan deposited in three and five layers on the surface. The images of FESEM, confocal microscopy, and AFM showed that the best performance of the LbL deposition process was after the surface modification with hexamethylenediamine (HMDA). The MTT assay showed that the presence of these biomolecules had boosted the proliferation of fibroblast cells. Increasing the number of the deposited layer from three to five, decreased the cell viability and antibacterial activity of the films. The obtained results propose the modification with HMDA and the deposition of three layers of collagen and chitosan on the PU films to improve its biocompatibility.