Premium
A biosensor platform based on amine functionalized conjugated benzenediamine‐benzodithiophene polymer for testosterone analysis
Author(s) -
Bulut Umut,
Sanli Serdar,
Cevher Sevki Can,
Cirpan Ali,
Donmez Sude,
Timur Suna
Publication year - 2020
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.49332
Subject(s) - cyclic voltammetry , biosensor , differential pulse voltammetry , polymer , glutaraldehyde , materials science , electrode , conductive polymer , detection limit , electrochemistry , contact angle , polymer chemistry , chemistry , chemical engineering , combinatorial chemistry , nanotechnology , organic chemistry , chromatography , composite material , engineering
A novel benzenediamine‐benzodithiophene polymer is synthesized for use in biosensor fabrication for the detection of testosterone. The sensory platform is constructed via drop coating on a screen‐printed carbon electrode, using poly(benzenediamine‐Bis[(2‐ethylhexyl)oxy]benzodithiophene) (pBDBT) as the polymer layer. Testosterone antibodies are immobilized on the polymer‐coated electrode surface via glutaraldehyde, which binds to the surface through the amino functional groups on the polymer backbone. The changes in the surface features due to testosterone binding are investigated via electrochemical techniques such as differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectrometry as well as contact angle measurements. Surface morphology of the modified electrodes is characterized by atomic force microscopy. The linear range and limit of detection of the sensor are calculated. Impact of possible interfering compounds is investigated. Furthermore, the sensory platform is utilized for testosterone analysis in synthetic biological fluids.