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Preparation of a composite monolith with functional graphene oxide and its application in the online enrichment of ursolic acid in medicinal plant
Author(s) -
Yu Huan,
Liu Haiyan,
Pang Xiaoya,
Zhang Miaomiao,
Yan Hongyuan,
Bai Ligai
Publication year - 2020
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.49081
Subject(s) - monolith , adsorption , desorption , composite number , chromatography , materials science , detection limit , graphene , ursolic acid , calibration curve , chemical engineering , ethylene oxide , chemistry , nuclear chemistry , copolymer , composite material , organic chemistry , nanotechnology , polymer , catalysis , engineering
Abstract In this study, a composite poly(functional graphene oxide‐ co ‐ N ‐isopropylacrylamide‐ co ‐trimethylolpropane triacrylate‐ co ‐ethylene dimethacrylate) (poly(f‐GO‐ co ‐NIPAAm‐ co ‐TMPTA‐ co ‐EDMA)) monolith was prepared, in which f‐GO was added. The obtained monolith was characterized by nitrogen adsorption–desorption isotherm and scanning electron microscopy. The monolith exhibited a homogeneous and continuous structure, good permeability, and large surface area. The composite monolith was used as solid‐phase extraction absorbent in conjunction with high‐performance liquid chromatography for enrichment of ursolic acid. The calibration equation was y = 73.3980 x + 0.2555, and the linear regression coefficient was 0.9987; The calibration curve was ranged from 1 to 700 μg ml −1 ; The limit of detection and limit of quantification values were 0.3 and 1.0 μg ml −1 , respectively; The intraday and interday precisions were less than 2.6 and 7.4%, respectively. The recovery was in the range of 84.44–111.76%. Moreover, the adsorption amount of ursolic acid on the composite monolith was compared with the monolith without f‐GO added and silica gel‐C18 adsorbent. The results showed that the composite monolithic column was feasible to be used as an online SPE absorbent, which exhibiting low back pressure, good permeability, and high adsorption capacity. In addition, this method provided the possibility to extract and analyze trace components from medicinal plants.