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Influence of pre‐electrophoresis on protein separations in polyacrylamide gels
Author(s) -
Haris Anfal,
Sanders Jonathan R.,
Arce Pedro E.
Publication year - 2020
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.48994
Subject(s) - acrylamide , electrophoresis , polyacrylamide , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , chromatography , free flow electrophoresis , gel electrophoresis , two dimensional gel electrophoresis , difference gel electrophoresis , chemistry , pulsed field gel electrophoresis , gel electrophoresis of nucleic acids , color marker , molecular weight size marker , polymer , monomer , polymer chemistry , biochemistry , proteomics , organic chemistry , enzyme , genotype , gene
Polyacrylamide gel electrophoresis (PAGE) is a widely used technique in protein separations. In this study, the effects of pre‐electrophoresis (i.e., exposing polyacrylamide gels to an electrical field prior to loading samples) on the separation of a mixture of 10 standard proteins was investigated. Three different gel concentrations (based on amounts of acrylamide and bis‐acrylamide, always at a 37.5:1 ratio) relative to the volume of the gel solution were tested: 6%, 9%, and 12%. For each concentration, multiple slab gels were subjected to pre‐electrophoresis prior to separation of proteins, while multiple other (i.e., companion) slab gels were used but without applying the pre‐electrophoresis technique. Separation results were investigated, showing that the application of the pre‐electrophoresis technique had a significant effect on separations particularly for 6% and 9% gels.